3/13/2024 0 Comments Tick identification njTicks have been incriminated as the second most important vectors of human and animal infectious pathogens in the world after mosquitoes and are able to transmit a wide range of pathogens, including bacteria, protozoans, viruses, and helminths. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. LNH received a grant of PhD scholarship from IHU Méditerranée Infection. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the manuscript and its Supporting Information files.įunding: This study was supported by the Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, the National Research Agency under the “Investissements d’avenir” programme, reference ANR-10-IAHU-03, the Région Provence Alpes Côte d’Azur and European ERDF PRIMI funding. Received: Accepted: SeptemPublished: September 28, 2021Ĭopyright: © 2021 Huynh et al. ![]() PLoS Negl Trop Dis 15(9):Įditor: Lynn Soong, University of Texas Medical Branch, UNITED STATES (2021) Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam. These findings suggested that ticks and tick-associated pathogens are likely to pose challenges to public and veterinary health in Vietnam.Ĭitation: Huynh LN, Diarra AZ, Pham QL, Le-Viet N, Berenger J-M, Ho VH, et al. The detected microorganisms that were confirmed by sequencing belonged to the Anaplasmataceae and Piroplasmida families. Our study revealed 97% correspondence between morphological and MALDI-TOF MS identification. This goal of this study was to assess the efficiency and reliability of MALDI-TOF MS for the identification of alcohol-preserved ticks collected in Vietnam and to determine the presence of their relative pathogens. This procedure represents a time-cost saving and does not require expert knowledge. ![]() Recently, MALDI-TOF MS has been used as an innovative tool for the rapid and accurate identification of alcohol-preserved ticks based on proteins from extracted legs. The identification of ticks and tick-associated diseases has an important role to play in epidemiological investigation and in assessing the risks of disease transmission to humans and animals. ![]() That said, serological and molecular evidence of tick infections in animals and humans have been reported on several occasions in Vietnam and Southeast Asia in recent decades. Nevertheless, studies on ticks and tick-borne infections remain limited in Vietnam. Ticks are one of the important vectors and reservoirs of multiple pathogens infecting humans and animals such as bacteria, protozoans, viruses, and helminths. The DNA of several microorganisms including Anaplasma platys, Anaplasma phagocytophilum, Anaplasma marginale, Ehrlichia rustica, Babesia vogeli, Theileria sinensis, and Theileria orientalis were detected in 25 ticks. The blind test of 310 spectra remaining after updating the database with 19 spectra revealed that all were correctly identified with log score values (LSV) ranging from 1.7 to 2.396 with a mean of 1.982 ± 0.142 and a median of 1.971. Spectral analysis showed intra-species reproducibility and inter-species specificity and the spectra of 329 (91%) specimens were of excellent quality. were submitted for MALDI-TOF MS analyses. In this study, the legs of 361 ticks collected in Vietnam, including 251 Rhiphicephalus sanguineus s.l, 99 Rhipicephalus ( Boophilus) microplus, two Amblyomma varanensis, seven Dermacentor auratus, one Dermacentor compactus and one Amblyomma sp. ![]() Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a promising and reliable tool for arthropod identification, including the identification of alcohol-preserved ticks based on extracted leg protein spectra.
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